Combination of His-tagged T4 endonuclease VII with microplate array diagonal gel electrophoresis for high-throughput mutation scanning.

نویسندگان

  • Matt J Smith
  • Gabriella Pante-de-Sousa
  • Khalid K Alharbi
  • Xiao-he Chen
  • Ian N M Day
  • Keith R Fox
چکیده

fied in the stalk region. J Biol Chem 2001;276:5525–32. 21. Okabe T, Fujisawa M, Yotsumoto H, Watanabe J, Takaku F, Lanzillo JJ, et al. Familial elevation of serum angiotensin-converting enzyme. Q J Med 1985; 55:55–61. 22. Luisetti M, Martinetti M, Cuccia M, Dugoujon M, De Rose V, Peona V, et al. Familial elevation of serum angiotensin-converting enzyme activity. Eur Resp J 1990;3:441–6. 23. Kramers C, Deinum J. Increased serum activity of angiotensin-converting enzyme (ACE): indication of sarcoidosis? A Bayesian approach. Ned Tijdschr Geneeskd 2003;147:473–6. 24. Linneback M, Kesper K, Jeub M, Urbach H, Wullner U, Klockgether T, et al. Hereditary elevation of angiotensin-converting enzyme suggesting neurosarcoidosis. Neurology 2003;61:1819–20. 25. Balyasnikova IV, Metzger R, Sun Z-L, Franke FE, Berestetskaya YV, Chubb AJ, et al. Monoclonal antibodies 1B3 and 5C8 as probes for monitoring the nativity of the soluble angiotensin-converting enzyme. Hybridoma 2005;24: 14–26. 26. Danilov S, Jaspard E, Churakova T, Towbin H, Savoie F, Wei L, et al. Structure-function analysis of angiotensin I-converting enzyme using monoclonal antibodies. J Biol Chem 1994;269:26806–14. 27. Kost OA, Balyasnikova IV, Chemodanova EE, Nikolskaya II, Albrecht RFII, Danilov SM. Epitope-dependent blocking of the angiotensin-converting enzyme dimerization by monoclonal antibodies to the N-terminal domain of ACE: possible link of ACE dimerization and shedding from the cell surface. Biochemistry 2003;42:6965–76. 28. Balyasnikova IV, Woodman ZL, Albrecht RFII, Natesh R, Acharya KR, Sturrock ED, et al. The localization of an N-domain region of angiotensinconverting enzyme involved in the regulation of ectodomain shedding using monoclonal antibodies. J Proteome Res 2005;4:258–67. 29. Daniels LB, Glew RH. -Glucosidase assays in the diagnosis of Gaucher’s disease. Clin Chem 1982;28(Pt 1):569–77. 30. Pilliquod Y, Reinharz A, Roth M. Studies on the angiotensin-converting enzyme with different substrates. Biochim Biophys Acta 1970;206:136– 42. 31. Friedland J, Silverstein E. A sensitive fluorometric assay for serum angiotensin-converting enzyme. Am J Pathol 1976;66:416–24. 32. Danilov SM, Savoie F, Lenoir B, Jeunemaitre X, Azizi M, Tarnow L, et al. Development of enzyme-linked immunoassays for human angiotensin I-converting enzyme suitable for large-scale studies. J Hypertens 1996;14:719– 27.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

SNP genotyping by combination of 192-well MADGE, ARMS and computerized gel image analysis.

A new modification of the microplate array diagonal gel electrophoresis (MADGE) system accommodates the dual amplification refractory mutation system (ARMS) products of 96 samples on one 192-well gel. Simultaneous electrophoresis of a number of horizontal ARMS-MADGE gels achieves high throughput. Gels are imaged digitally, here using the FluorImager 595 fluorescent scanning system. Customized s...

متن کامل

Microplate array diagonal gel electrophoresis for cohort studies of microsatellite loci.

After PCR amplification, we have achieved precise sizing of trinucleotide and tetranucleotide microsatellite alleles on 96-well open-faced polyacrylamide microplate array diagonal gel electrophoresis (MADGE) gels: two tetranucleotide repeats, HUMTHOI (five alleles 248-263 bp) and DYS390 (eight alleles 200-228 bp), and DYS392, a trinucleotide repeat (eight alleles 210-231 bp). A gel matrix of Du...

متن کامل

High-throughput method for determination of apolipoprotein E genotypes with use of restriction digestion analysis by microplate array diagonal gel electrophoresis.

Molecular epidemiological research has identified the association of a common apolipoprotein E (apo E) isoform (E4 as opposed to E3), with risk both of coronary artery disease and of Alzheimer dementia. In addition, the role of apo E genotype (usually E2/E2) in Type III hyperlipidemia is well known. However, both for diagnostic and research purposes, apo E genotyping is cumbersome. The preferre...

متن کامل

Typing dinucleotide repeat loci using microplate array diagonal gel electrophoresis: proof of principle.

Polymorphic dinucleotide repeat loci ('microsatellite markers') are found in varying abundance throughout the genomes of most organisms. They have been extensively used for genetic studies, but conventional techniques used for their genotyping require sophisticated equipment. Microplate array diagonal gel electrophoresis (MADGE) has previously been extended to economical high-throughput genotyp...

متن کامل

Manual 768 or 384 well microplate gel 'dry' electrophoresis for PCR checking and SNP genotyping.

Electrophoresis continues to be a mainstay in molecular genetic laboratories for checking, sizing and separating both PCR products, nucleic acids derived from in vivo or in vitro sources and nucleic acid-protein complexes. Many genomic and genetic applications demand high throughput, such as the checking of amplification products from many loci, from many clones, from many cell lines or from ma...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 51 6  شماره 

صفحات  -

تاریخ انتشار 2005